ECTS2014 Poster Presentations Bone development/growth and fracture repair (55 abstracts)
1,25-Dihydroxy vitamin D assay with on-board sample purification on the IDS-iSYS automated system
Jacqueline Tran , Dianne Bautista , Zoltan Seres , Loic Cornaut , Torquil Gundlach , Alain Rousseau & Hans-Werner Griesser
Immunodiagnostic Systems (IDS) Ltd., Boldon, UK.
1,25-Dihydroxyvitamin D (1,25D) is one of the major regulators of calcium metabolism. Due to its lipophilic nature and low circulating concentration, the measurement of 1,25D concentration levels has been labour intensive and technique dependent in addition to multiple equipments required for the sample purification procedure. We reported the results of soon to be commercialised fully on-board IDS-iSYS 1,25 VitDXp assay.
IDS-iSYS 1,25 VitDXp assay purifies the human sera utilising the anti-1,25D antibody coated magnetic particles to capture 1,25D in cuvette 1. After incubation, the magnetic particles are washed and 1,25D is eluted. The eluate is transferred to cuvette 2 where the immunoassay procedure will take place, using the currently available IDS-iSYS 1,25-dihydroxy vitamin D test. Below are the preliminary analytical performance of the IDS-iSYS 1,25 VitDXp (iSYS XP125) assay:
Combining the innovative on-board sample purification procedure with the already proven IDS-iSYS 1,25-dihydroxy vitamin D test, the IDS-iSYS 1,25 VitDXp not only enhances the clinical laboratory 1,25D testing efficiency, it also delivers accurate results for patient care.
| Performance | Result |
| Inter-assay precision | 9.8% (18.7 pg/ml) |
| 8.3% (59.3 pg/ml) | |
| 5.8% (95.5 pg/ml) | |
| 6.1% (163.9 pg/ml) | |
| Linearity | 90–113% |
| Method comparison against current IDS-iSYS 125D (n=121) | ISYS XP125=0.99×(iSYS 125D)−0.75 pg/ml r=0.97 |
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