In vitro inhibitory activity on RANKL-mediated osteoclastogenesis of Glossogyne tenuifolia extract

Shih-Wei Wang; Han-Chun Kuo; Hsia-Fen Hsu; Yuan-Kun Tu; Tien-Tsai Cheng; Jer-Yiing Houng

doi:10.1530/boneabs.3.PP172

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Bone Abstracts (2014) 3 PP172 | DOI: 10.1530/boneabs.3.PP172

ECTS2014 Poster Presentations Cell biology: osteoclasts and bone resorption (22 abstracts)

In vitro inhibitory activity on RANKL-mediated osteoclastogenesis of Glossogyne tenuifolia extract

Shih-Wei Wang ^1, , Han-Chun Kuo ^1, , Hsia-Fen Hsu ³ , Yuan-Kun Tu ⁴ , Tien-Tsai Cheng ⁵ & Jer-Yiing Houng ^1,

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¹Institute of Biotechnology and Chemical Engineering, I-Shou University, Kaohsiung, Taiwan; ²Department of Internal Medicine, Division of Allergy, Immunology, and Rheumatology, E-DA Hospital, Kaohsiung, Taiwan; ³Department of Nutrition, I-Shou University, Kaohsiung, Taiwan; ⁴Department of Orthopedics, E-Da Hospital, Kaohsiung, Taiwan; ⁵Department of Internal Medicine, Division of Rheumatology, Allergy and Immunology, Chang Gung Memorial Hospital – Kaohsiung Medical Center, Chang-Gung University College of Medicine, Kaohsiung, Taiwan.

Receptor activator of nuclear factor kappa B ligand (RANKL)-induced activation of NF-κB and MAPKs signaling pathways is critical to osteoclastogenesis. Glossogyne tenuifolia has been used as an antipyretic, detoxication, and anti-inflammatory herb tea in Penghu Island, Taiwan. This study investigated the effects of G. tenuifolia ethanolic extract (GTE) on the formation of RANKL-activating osteoclasts and expression of signaling pathways using a RAW264.7 cells model. GTE significantly inhibited the RANKL-induced formation of TRAP-positive multinucleated osteoclasts in a dose-dependent manner. GTE inhibited RANKL-induced activation of nuclear factor of activated T-cells (NFATc1) and suppressed the phosphorylation of NF-κB/p65 and JNK. Additionally, GTE reduced the phosphorylated forms of osteoclast survival-related signaling molecules, including NF-κB, JNK, p38, and Akt. Taken together, these results reveal that GTE may have potential in the development of health food against osteoclast-related diseases such as osteoporosis.