ECTS2014 Poster Presentations Arthritis and other joint diseases: translational and clinical (15 abstracts)
RANK expression is reduced in circulating monocytes from ankylosing spondylitis patients
Inês Pedro Perpetuo 1 , Joana Caetano-Lopes 1 , Elsa Vieira-Sousa 1, , Raquel Campanilho-Marques 1, , Cristina Ponte 1, , Helena Canhao 1, , Mari Ainola 3 & Joao Eurico Fonseca 1,
1JE Fonseca Lab (Rheumatology Research Unit), Instituto de Medicina Molecular, Lisbon Academic Medical Centre, Faculdade de Medicina da Universidade de Lisboa, Lisboa, Portugal; 2Rheumatology and Bone Metabolic Diseases Department, Lisbon Academic Medical Centre, Hospital de Santa Maria, Centro Hospitalar Lisboa Norte, EPE, Lisboa, Portugal; 3Musculoskeletal Diseases and Inflammation Research Group, Biomedicum Helsinki, Faculty of Medicine, Institute of Clinical Medicine, University of Helsinki, Helsinki, Finland.
Rheumatoid arthritis (RA) and ankylosing spondylitis (AS) are systemic, immune-mediated diseases. RA’s main targets are the peripheral joints while AS has the axial skeleton and enthesis as the principal affected areas. RA is characterized by bone erosions and impaired repair whilst AS is typified by bone overgrowth. The causes for these differences are not yet understood; however we hypothesize that AS patients’ monocytes receive reduced osteoclastogenic stimuli and/or have abnormal capacity to respond to them.
The aim of this study was to characterize AS patients’ bone remodeling and pro osteoclastogenesis inflammatory environment and monocyte phenotypes as compared to RA patients and healthy controls.
Untreated AS or RA patients with active disease and age and gender-matched healthy donors were recruited for this study. Blood was collected for flow cytometry measurements of RANKL expression and monocyte subpopulation characterization. Serum was collected for cytokine and bone remodeling factors quantification. This study was approved by the hospital’s ethics committee.
We found that RANKL lymphocyte expression was higher in AS and RA patients when compared to healthy donors. However, both in classical and intermediate monocyte subpopulations RANK expression was lower in AS patients as compared to RA patients and healthy controls. In accordance, CTX-I serum level was also lower in AS patients than in healthy controls.
Conclusion: Despite comparable osteoclastogenic stimuli in AS and RA patients, RANK expression is reduced in AS circulating monocytes which may contribute to the bone forming phenotype observed in AS patients.
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ISSN 2052-1219 (online)
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