ECTS2013 Poster Presentations Bone development/growth and fracture repair (40 abstracts)
The retention and bioactivity of rhBMP-2 released from a bisphosphonate-linked hyaluronan-based hydrogel
Gry Hulsart-Billström 1 , Pik-Kwan Yuen 1 , Richard Marsell 1 , Jöns Hilborn 2 , Sune Larsson 1 & Dmitri Ossipov 2
1Department of Orthopedics, Uppsala University, Uppsala, Sweden; 2Division of Polymer Chemistry, Department of Materials Chemistry, Uppsala University, Uppsala, Sweden.
Introduction: There are several disadvantages with the present carriers used in rhBMP-2 products including risk for immunological response, inefficient release and poor handling properties. Our aim was to examine the release of rhBMP-2 from a bisphosphonate-linked hyaluronan hydrogel with the hypothesis that it would cause a slower release.
Methods: Triplicates of hydrogels with rhBMP-2 and with (HA-BP) or without (HA) bisphosphonate were prepared, after which cell culture medium was added and refreshed at 1, 3, 6, 12, 24, 48, and 72 h and day 6, 9, 12, and 14. The extracts were analysed using ELISA, after which the gels were degraded and analyzed by ALP-assay on stromal-cells.
Results: 6% of rhBMP-2 had been released from the HA-BP hydrogel at the end of the study, compared with 100% from the HA hydrogel. The retained rhBMP-2 induced ALP expression of stromal-cells.
Discussion and conclusions: Bisphosphonate caused a remarkable slower release of rhBMP-2 when linked to the hydrogel. The biological functions of the retained rhBMP-2 were still intact.
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