ECTS2013 Poster Presentations Other diseases of bone and mineral metabolism (48 abstracts)
Long-term energy deficiency in mice induces bone alterations reversed by long-term recovery
Sara Zgheib 1 , Stéphanie Lucas 1 , Mathieu Mequinion 2 , Odile Broux 1 , Damien Leterme 1 , Pierre Hardouin 1 , Odile Viltart 2, & Christophe Chauveau 1
1Physiopathologie des Maladies Osseuses Inflammatoires, EA4490, ULCO-Lille 2, Boulognesur Mer, Lille, Nord-Pas de Calais, France; 2Développement et Plasticité du Cerveau Postnatal, UMR837 Inserm, JPARC, Lille, Nord-Pas de Calais, France; 3Université de Lille 1, Lille, Nord-Pas de Calais, France.
Anorexia nervosa (AN) a condition of profound undernutrition, is characterized by alterations in neuroendocrine and metabolic functions. Among the serious pathological consequences of this eating disorder, osteoporosis is often observed and persists after recovery, leading to a high fracture risk.
To study particularly bone alterations and recovery, a long term mouse model has been developed. In this model named separation-based anorexia (SBA) – a chronic stress induced by separation is associated with a restricted-time feeding schedule. Eight-week-old C57Bl/6J females were separated and their food access was gradually reduced from 6 to 2 h/day (SBA). After 10 weeks mice were housed again in standard conditions for 10 more weeks (recovery).
During the first 2 weeks of the SBA protocol, mice lost 25% of their initial body weight and then maintained this underweight while eating only 10% less than control mice. Fat and lean masses were quickly decreased and bone mineral acquisition mass was disrupted. Cortical and trabecular bone mineral densities of the tibia were significantly reduced. Reproductive functions were also rapidly and strongly altered and mice were hypoleptinemic.
The recovery phase allowed a rapid normalisation of body weight, fat and lean masses as well as reproductive functions. After 10 weeks of the recovery phase, all the mice had similar bone mineral content, but SBA mice still exhibited low leptinemia despite their recovered fat mass.
We hypothesised that the high capacity of bone normalization of recovered mice could be linked to this specific context of persisting hypoleptinemia associated with normalization of the other parameters.
Article tools
My recent searches
My recently viewed abstracts
Authors
Bone Abstracts
ISSN 2052-1219 (online)
© Bioscientifica 2024 |
Privacy policy |
Cookie settings
BiosciAbstracts
Bioscientifica Abstracts is the gateway to a series of products that provide a permanent, citable record of abstracts for biomedical and life science conferences.
Find out more