Bone Abstracts

It is well known that sex hormone deficiency leads to increased bone turnover and subsequent bone loss. The metabolism of estrogens involves, among others, oxidation (mainly hydroxylation) by CYPs. The aim of this study was to determine whether Leu432Val polymorphism in the CYP1B1 gene is present also in Slovak population and subsequently, if it is associated with femoral and spinal bone mineral density (FBMD and SBMD), bone remodeling markers and fracture incidence in this population.The study sample consisted of 338 postmenopausal women (63.4±7.5 years) including osteoporotic, osteopenic, and healthy individuals. Subjects were selected according to strict inclusion criteria. Genotypes were detected using PCR–RFLP method. CYP1B1 genotypes and allele frequencies were tested using the chi-square test. The differences between the genotypes were analyzed by GLM procedure and covariance analysis after correction of the measurements for age and BMI. In the analyzed Slovak population we calculated the distribution of genotypes of CG (46.7%), CC (34.6%), and GG (18.6%), where C allele disposed higher frequency (0.58). We didn’t find a relationship between Leu432Val polymorphism and femoral and spinal BMD. Within the association study of CYP1B1 genotypes with bone formation (ALP and OC) and bone resorption markers (Ctx), we found no overall association among analyzed postmenopausal women, as well as fracture incidence (P>0.05). Our data reveal no association between Leu432Val polymorphism and parameters of bone turnover markers and FBMD, SBMD in a population of Slovak postmenopausal women. The results can contribute to a comprehensive view of the genetics of osteoporosis. All procedures were approved by the Ethical Committee of the Specialized Hospital of St Svorad in Nitra. This work was supported by grants KEGA 025UKF-4/2012; 035UKF-4/2013.