ECTS2013 Poster Presentations Chondrocytes and cartilage (20 abstracts)
Effects of an in vitro low-oxygen-tension preconditioning of adipose stem cells on their in vivo chondrogenic potential: application in cartilage tissue repair
Sophie Portron 1, , Christophe Merceron 1, , Olivier Gauthier 1, , Julie Lesoeur 1, , Sophie Sourice 1, , Martial Masson 1, , Borhane Fellah 1, , Olivier Geffroy 1, , Elodie Lallemand 1, , Pierre Weiss 1, , Jérôme Guicheux 1, & Claire Vinatier 1,
1INSERM, UMRS 791, Center for Osteoarticular and Dental Tissue Engineering, Group STEP ‘Skeletal Tissue Engineering and Physiopathology’, Nantes, France; 2University of Nantes, UFR Odontology, Nantes, France; 3Center for Preclinical Research and Investigation of the ONIRIS Nantes-Atlantic College of Veterinary Medicine, Food Science and Engineering (CRIP), Nantes, France; 4Department of Equine Surgery, College of Veterinary Medicine of Nantes (ONIRIS), Nantes, France.
Purpose: Multipotent stromal cells (MSC)-based regenerative strategy is promising for the repair of cartilage, which is an avascular tissue in which cells experience hypoxia. Hypoxia is known to promote the early chondrogenic differentiation of MSC. Therefore, the aim of our study was to determine whether low oxygen tension could be used to enhance the regenerative potential of MSC for cartilage repair.
Methods: MSC from rabbits or human adipose tissues (ASC) were preconditioned in vitro in control or chondrogenic (ITS and TGFβ) medium and in 21 or 5% O2. Chondrogenic commitment was monitored by measuring COL2A1 and ACAN expression level by real-time PCR. Preconditioned rabbit and human ASC were then incorporated in a Si-HPMC hydrogel and respectively injected i) in rabbit articular cartilage defects for 18 weeks or ii) subcutaneously in nude mice for 5 weeks. The newly formed tissue was qualitatively and quantitatively evaluated by cartilage-specific immunohistological staining (Alcian Blue, type II collagen) and scoring (O’Driscoll score). The phenotype of ASC cultured in a monolayer or within Si-HPMC in control or chondrogenic medium and in 21 or 5% O2 was finally evaluated using real-time PCR.
Results/Conclusions: 5% O2 increased the in vitro expression of chondrogenic markers in ASC cultured in induction medium. The cells implanted within the Si-HPMC hydrogel and preconditioned in chondrogenic medium formed a cartilaginous tissue, regardless of the level of oxygen. In addition, the 3D in vitro culture of ASC within the Si-HPMC hydrogel was found to reinforce the prochondrogenic effects of the induction medium and 5% O2. Altogether, these data indicate that although 5% O2 enhances the in vitro chondrogenic differentiation of ASC, it does not enhance their in vivo chondrogenesis. These results also highlight the in vivo chondrogenic potential of ASC and their potential value in cartilage repair.
Article tools
My recent searches
My recently viewed abstracts
Authors
Bone Abstracts
ISSN 2052-1219 (online)
© Bioscientifica 2024 |
Privacy policy |
Cookie settings
BiosciAbstracts
Bioscientifica Abstracts is the gateway to a series of products that provide a permanent, citable record of abstracts for biomedical and life science conferences.
Find out more