ECTS2013 Poster Presentations Cancer and bone: basic, translational and clinical (31 abstracts)
Cytotoxicity of picocyanobacteria strains of the genera Cyanobium on osteosarcoma cells
Rosário Martins 2, , Margarida Costa 3 , Mónica Garcia 1 , Piedade Barros 2 , João Costa-Rodrigues 1 , Vitor Vasconcelos 3, & Maria Fernandes 1
1Laboratory for Bone Metabolism and Regeneration, Faculdade de Medicina Dentária, Universidade do Porto, Porto, Portugal; 2CISA – Centro de Investigação em Saúde e Ambiente, Escola Superior de Tecnologia da Saúde do Porto, Instituto Politécnico do Porto, Porto, Portugal; 3CIIMAR – Centro Interdisciplinar de Investigação Marinha e Ambiental, Universidade do Porto, Porto, Portugal; 4Faculdade de Ciências, Universidade do Porto, Porto, Portugal.
Marine cyanobacteria have been recognized as an important source of bioactive compounds. The cytotoxicity on cancer cell lines has been extensively explored and several cyanobacteria metabolites are already described as potential anticancer compounds or are considered useful templates for the design of new anticancer drugs. The majority of compounds have been isolated from filamentous or colonial cyanobacteria that growth in high densities along shores. In contrast, picoplanktonic forms have rarely been explored since, for these strains, there is a need for culture for biomass production. From our LEGE cyanobacteria culture collection we selected a panel of seven strains of the picocyanobacteria genera Cyanobium in order to explore it’s potential as anticancer agents. Strains were cultured under laboratory conditions. Freeze-dried biomass was extracted using methanol and dichloromethane to a crude extract and then fractionated using hexane, ethyl acetate and methanol. The cytotoxicity of crude extracts and fractions was evaluated in the osteosarcoma cell line MG63 by the reduction of the bromide 3-(4,5-dimetil-tiazol-2-il)-2,5-difenil-tetrazolio (MTT) and confirmed by the lactate dehydrogenase (LDH) assay. From the results, four of the seven Cyanobium strains were found to induce a significant decrease in cell viability. The highest percentage of inhibition of tumor cells growth was observed within the ethyl acetate, which is therefore, promising in terms of isolation of bioactive compounds.
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