ECTS2013 Poster Presentations Calciotropic and phosphotropic hormones and mineral metabolism (33 abstracts)
Pro-angiogenic and pro-survival functions of glucose in human mesenchymal stem cells upon transplantation
Mickael Deschepper , Joseph Paquet , Mathieu Manassero , Morad Bensidhoum , Karim Oudina , Delphine Logeart-Avramoglou & Herve Petite
B2OA, Paris, France.
A major limitation in the development of cellular therapies using human mesenchymal stem cells (hMSCs) is cell survival post-transplantation. In this study, we challenged the current paradigm of hMSC survival, which assigned a pivotal role to oxygen, by testing the hypothesis that exogenous glucose may be key to hMSC survival. We demonstrated that hMSCs could endure sustained near-anoxia conditions only in the presence of glucose. In this in vitro cell model, the protein expressions of Hif-1α and angiogenic factors were up-regulated by the presence of glucose.
Ectopically implanted tissues constructs supplemented with glucose exhibited four- to fivefold higher viability and were more vascularized compared to those without glucose at day 14. These findings provided the first direct in vitro and in vivo demonstration of the pro-angiogenic and pro- survival functions of glucose in hMSC upon transplantation and identified glucose as an essential component of the ideal scaffold for transplanting stem cells.
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