ECTS2013 Poster Presentations Chondrocytes and cartilage (20 abstracts)
Prenatally administered acrylamide programs a gut-bone axis of guinea pig newborns
Ewa Tomaszewska 1 , Piotr Dobrowolski 2 , Paulina Kurlak 2 , Barbara Badzian 1 , Monika Hulas-Stasiak 2 , Iwona Puzio 1 & Krzysztof Kostro 1
1University of Life Sciences in Lublin, Lublin, Poland; 2Maria Curie-Sklodowska University, Lublin, Poland.
Acrylamide is a byproduct that forms when certain carbohydrate and aminoacid rich foods are fried, baked, or roasted at high temperatures (>120°C). Our earlier study showed that acrylamide altered the morphology and histology of the small intestinal wall damaging the intestinal barrier and reducing absorption surface. The study was performed to determine whether acrylamide influences gut-bone axis in foetus when administered to guinea pig during the last 35 days of pregnancy. The study was carried out on newborns born by guinea pigs receiving clear tap water to drink (the control group, n=6) and by guinea pigs receiving acrylamide in water do drink in the dose of 3 mg/kg BW per day (the Ac group, n=6). The amount of acrylamide in water was adjusted daily, according to weight increase of pregnant guinea pigs, to achieve appropriate dose. The histomorphometry of growth plate and articular cartilage of tibia as well as small intestine wall was determined. Immunohistochemical staining with anti cadherin antibodies was performed to mark adherent type cell–cell junctions in small intestine epithelium. Lowered expression for cadherin was found in the duodenum and middle part of jejunum in the Ac group of newborn offspring. Acrylamide administration significantly reduced the thickness of the hypertrophy and calcified zones of growth plate, and thickened the radial zone of articular cartilage of tibia. Present study showed that acrylamide might influence development and mineralization of bones during prenatal time by disturbance of gut-bone axis.
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