Bone Abstracts

Objectives: The aim of this study was establish features changes of organometric parameters of bones in mature male rats after a 60-day inhalation seed toluene.

Materials and methods: For the experiment were selected 60 mature male rats were divided into two groups: 1st – intact rats, 2nd group – the rats that every day for 2 months to device for inhalation agents received a one-time inhalation of toluene exposure 4 h in 10 maximum permissible concentration. Periods of experiment were 1, 7, 15, 30, and 60 days. Rats were euthanized under ether mask anesthesia after the end of the 2-month impact of toluene. Tibia, hip bone and the third lumbar vertebra were taken and measured by caliper.

The results: The maximum length of the tibia and hip bone were lower than in 1st group from 7 to 60 day at 4.15, 4.47, 2.67, 3.35, 3.41 and 3.84, 3.78, 4.88, 4.16, 2.91% respectively. Height body of third lumbar vertebra was also less than the control, respectively, at 5.12, 6.27, 6.69, 3.28, and 4.80%. The width of the proximal and distal tibial epiphysis decreased in the same period at 3.69, 4.14, 5.77, 4.49, 2.84 and 11.94, 7.78, 10.75, 10.00, and 6.86% and the width and the anterior–posterior size of the middle part of diaphysis – at 6.82, 6.72, 7.01, 5.82, 6.05 and to 12.31, 10.14, 9.80, 8.65, 7.11%. The maximum width of the hip bone was lower than in intact animals from 7 to 60 day at 6.06, 6.19, 6.89, 4.87, and 5.53%, and the width body of third lumbar vertebra – at 7.27, 7.12, 5.90, 5.43, and 5.69%.

Conclusions: Inhaled seed toluene followed by a slowdown in both longitudinal and appositional bone growth. Identified changes manifested by 1, 7, and 15 days after the end of inhalation seed toluene with a tendency to leveling from 30 to 60 days.