ECTS2013 Poster Presentations Bone development/growth and fracture repair (40 abstracts)
The effect of incubation time of preformed injectable hydrogels on bone formation when used as carrier of rhBMP-2
Sonya Piskounova 2 , Gry Hulsart-Billström 1 , Lars Gedda 3 , Kristoffer Bergman 2 , Jöns Hilborn 2 , Sune Larsson 1 & Tim Bowden 2
1Department of Orthopedics, Uppsala University, Uppsala, Sweden; 2Ångström Laboratory, Department of Chemistry, Uppsala University, Uppsala, Sweden; 3Department of Oncology, Radiology and Radiation Science, Uppsala University, Uppsala, Sweden.
Introduction: Hydrogels has demonstrated efficacy as carriers for growth factors. Our aim was to investigate the effect of curing-time of modified hyaluronan on bone formation.
Methods: Hydrogels with rhBMP-2 were cross-linked for 14 and 3 days, 5 h or 1 min before injection. Preformed gels were injected s.c. in 5 rats, the rats were killed after 5 weeks. Explanted samples were radiographed and scanned by pQCT.
Results: Bone formation occurred in all samples. Radiographs revealed higher attenuation for the 5 h cross-linked hydrogel. The same result was seen in the pQCT were both 5 h and 1 min preformed hydrogels had significantly higher bone density compared to 3 days (P=0.0064, ANOVA Tukey’s multiple comparison test; Fig. 1). 5 h yielded higher bone mineral content compared to 1 min cross-linked gel (P=0.0116 ANOVA Tukey’s multiple comparison test).
Conclusion: A minimum of 5 h curing-time gives the most efficient bone formation concerning density, mineral content and volume.
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